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Bio-Techne corporation
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Cell Signaling Technology Inc
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Welgen Inc
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Welgen Inc
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Image Search Results
Journal: Molecular cell
Article Title: Lipid droplet-derived monounsaturated fatty acids traffic via PLIN5 to allosterically activate SIRT1
doi: 10.1016/j.molcel.2019.12.003
Figure Lengend Snippet: A) Saturation plot of SIRT1 activity towards FOXO3a and the effects of 18:1 and resveratrol (n=4). B) Lineweaver-Burk reciprocal plots were generated to determine Km, Vmax, and Kcat for the FOXO3a peptide substrate. C-D) Km and Kcat/Km fold change for each concentration of 18:1 on FOXO3a. E) Kcat/Km fold change for resveratrol (Res; 10 μM). F) Saturation plot of SIRT1 activity towards H3 and the effects of 18:1 and resveratrol. G) Lineweaver-Burk reciprocal plots for the H3 peptide substrate. H-I) Km and Kcat/Km fold change for each concentration of 18:1 with H3. J-K) Kcat/Km fold change for each concentration of resveratrol and fatty acids for the H3 peptide substrate. L) Competition assay of SIRT1 activity towards FOXO3A, PGC-1α and H3 acetylated peptide substrates.
Article Snippet: Antibodies were purchased or obtained from the following sources; Total-Plin5 (Progen; Heudelberg, Germany),
Techniques: Activity Assay, Generated, Concentration Assay, Competitive Binding Assay
Journal: Molecular cell
Article Title: Lipid droplet-derived monounsaturated fatty acids traffic via PLIN5 to allosterically activate SIRT1
doi: 10.1016/j.molcel.2019.12.003
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Antibodies were purchased or obtained from the following sources; Total-Plin5 (Progen; Heudelberg, Germany),
Techniques: Recombinant, Reporter Assay, Mutagenesis, Software, Derivative Assay